Abstract
A sensitive, accurate, simple, and rapid analytical LC–MS/MS method was developed for the identification and quantification of koumine, gelsemine, and gelsenicine in human hair. Approximately 10 mg of hair was extracted with methanol by cryogenic grinding. The limits of detection (LODs) ranged from 1 to 5 pg/mg, and the limits of quantitation (LOQs) ranged from 2 to 10 pg/mg. The method was linear over a concentration range from the LOQs to 10000 pg/mg, with the linear correlation (R2) of the calibration curves for all analytes above 0.998. The bias varied from -3.6 to 8.8%, while the intra- and inter-day precision Relative Standard Deviation (RSD) values ranged from 1.8–10.8% and 1.9–9.6%, respectively. Recoveries were within the range of 77.8% to 83.3%, and matrix effects were in the range of 74.3% to 87.4%. The present method was suitable for quantitative determination of koumine, gelsemine, and gelsenicine in human hair from one Gelsemium elegans poisoning case. The highest concentrations of koumine, gelsemine, and gelsenicine in the hair segment corresponding to the period of ingestion were 29.4, 17.6, and 4.7 pg/mg, respectively. To our knowledge, this study is the first to describe hair analysis in a Gelsemium poisoning case and to provide quantitative toxicological findings.
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