Confirmation of adherence Protein Hemagglutinin sub-unit Pili with MW 49.6 kDa Helicobacter pylori on Mice Gastric Epithelial Cell

Abstract

Helicobacter pylori (H.pylori) is a gram-negative microaerophilic bacterium that is generally associated with the main cause of approximately 80% peptic ulcers in stomach. It is also linked to the development of the stomach cancer. The aim of this study was to characterize the sub-unit pili proteins of Helicobacter pylori with a molecular weight of about 49,6 kDa as a haemagglutinin protein and adhesion molecules on surface of mice gastric epithelial cells. The bacterium was firstly cultured on the plate of TSA-B (Trypticase Soy Agar with 5% Sheep Blood) to prepare the protein of interes using bacterial cutter. The isolated proteins were subsequently analyzed for haemagglutination capability using mice sentisized erithrocytes. SDS- PAGE and western blotting analyses were used to confirm the protein patterns and their immunological reactivities respectively. The both SDS-PAGE and western blotting analyses showed that a dominant protein with molecular weight of about 49,6 kDa was detected consistently and found to be potentially aglutinate the erythrocytes. Furthermore, this protein was observed to be adherence on mice gastric epithelial cells. This study has indicated that the sub-unit pili proteins of H. pylori with a molecular weight of about 49,6 kDa was found to be dominant and immunogenic. Further study is required to confirm the in vivo properties of the protein associated with pathogenesis of H. pylori infection.