Confining Prepared Ultra-Small Nanozymes Loading Ato for Lung Cancer Catalytic Therapy/Immunotherapy.

Abstract

Nanozymes with inherent enzyme-mimic catalytic properties combat malignant tumor progression via catalytic therapy, while the therapeutic efficacy still needs to be improved. In this work, we innovatively synthesized ultra-small platinum nanozymes (nPt) in confined domain of wormlike pore channel in gold nanobipyramidal-mesoporous silica dioxide nanocomposites, producing nanozyme carriers AP-mSi with photo-enhanced peroxidase ability. Afterwards, based on the prepared AP-mSi, the lung cancer nanozymes probe (AP-HAI) was ingeniously produced by removing SiO2 template, modifying HSA, and loading ATO as well as IR780. Under NIR light irradiation, inner AuP and IR780 collaborated for photothermal process, thus facilitating the peroxidase-like catalytic process of H2 O2 . Additionally, loaded atovaquone molecules (ATO), a cell respiration inhibitor, could impair tumor respiration metabolism and cause oxygen retention, hence enhancing IR780's PDT effectiveness. In a result, IR780's PDT property and nPt nanozymes' photo-enhanced peroxidase-like ability endowed probes a high ROS productivity, eliciting anti-tumor immune responses to destroy tumor tissue. Systematic studies revealed that the obvious ROS generation was obtained by the strategy of using nPt nanozymes and reducing oxygen consumption by ATO, which in turn enabled lung cancer synergetic catalytic therapy/ICD-based immunotherapy. The results of this work would provide theoretical justification for the practical use of photo-enhanced nanozyme probes. This article is protected by copyright. All rights reserved.