Cones contribute to light-evoked, dopamine-mediated uncoupling of horizontal cells in the mudpuppy retina.

Abstract

1. The relative effectiveness of adapting lights of different wave-lengths on uncoupling of horizontal cells was measured in dark-adapted mudpuppy retinas. Diffuse blue (470 nm) or red (620 nm) adapting stimuli were adjusted in intensity to be equally effective for rods or for cones. Uncoupling of horizontal cells was measured by intracellular recording of changes in their responses to spot and annulus stimuli. The intensities of the adapting light pairs were varied over 3 log units. The responses of the horizontal cells indicated that both rods and cones were stimulated by the adapting lights. 2. Relatively dim adapting lights did not produce detectable changes in horizontal cell coupling. Brighter adapting lights caused uncoupling of horizontal cells. When the brighter adapting lights were rod matched, the uncoupling effect of the 620-nm light was significantly greater than that of the 470 nm light, indicating that cones contribute to the uncoupling effect. 3. When the adapting lights were cone matched, the effects of the two wavelengths were not significantly different, but this did not rule out a rod contribution because the effective adapting lights probably produced maximal or nearly maximal, and hence equal or nearly equal, responses in rods. 4. The results indicate that cones contribute to the light-evoked uncoupling of horizontal cells in mudpuppy, although a contribution from rods could not be ruled out. Because it was shown previously that light-evoked uncoupling of horizontal cells in mudpuppy is mediated by dopamine, the results also suggest that cones contribute to the light-evoked release of dopamine.