Conducting microporous organic polymer with –OH functional groups: Special structure and multi-functional integrated property for organophosphorus biosensor

Abstract

The potential application of microporous organic polymers (MOPs) in various fields have been demonstrated. However, further study of MOPs based on novel idea and delicate design remain a challenge. Herein, a unique phloroglucinol-based MOP with large numbers of –OH functional groups (OH-POF) was synthesized via simple and cost-effective microwave synthesis. Interestingly, the π-π interaction and hydrogen bonding among OH-POF, acetylcholinesterase (AChE), and Nafion (NF) are beneficial to the combination of modification materials and immobilized enzymes into a whole on the surface of electrode. Furthermore, special structure and multi-functional integrated OH-POF material with a hierarchical pore system and large specific surface area as well as excellent electrical conductivity was applied as the immobilizations of AChE on carbon paste electrode (CPE) for improving its catalysis ability to construct organophosphate pesticides (OPs) biosensor. The electrochemical analysis of paraoxon and methyl parathion was achieved with wide linear range (5.0 × 10−13–1.0 × 10−8 g∙mL−1 for methyl parathion, 1.0 × 10−13–1.0 × 10−9 g∙mL−1 for paraoxon) and low limit of detection (LOD) (1.5 × 10−13 g∙mL−1 for methyl parathion, 3.4 × 10−14 g∙mL−1 for paraoxon). This work demonstrates the potential value of OH-POF material for electrochemical biosensor. Most importantly, it provides a fresh research idea and reference of further exploring the application of other MOPs in the field of environmental analysis.