Abstract
The rate of petite induction of an adenine-requiring Saccharomyces cerevisiae was strongly inhibited by the presence of 10% glucose or nalidixic acid (500 μg/ml) during the incubation in minimal medium + adenine + 0.5% glucose + ethidium bromide. The presence of chloramphenicol or the absence of adenine had no influence on the induction rate. The petite induction rate was also strongly inhibited when the minimal medium + adenine + 0.5% glucose was replaced by phosphate buffer + adenine + 0.1% glucose. We conclude that under some conditions mtDNA can replicate normally in the presence of ethidium.
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