Abstract

Two phenomena hamper the quantification of ROESY intensities: the off-resonance effect and the coherent magnetization transfer between scalar-coupled spins. The off-resonance application of the r.f. irradiation has been proposed to reduce these artefacts. Here the reliability of this method for the quantification of ROESY intensities measured on a sample containing a macromolecule was investigated. The threshold of irradiation offset for which the coherent magnetization transfer can be neglected was determined. It is shown that for offsets larger than this threshold, the off-resonance effect does not hinder the quantification, provided that the irradiation is alternatively applied at high and low field. © 1998 John Wiley & Sons, Ltd.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.