Abstract
When cattle oocytes are fertilized through intracytoplasmic sperm injection, the sperm is injected directly into the cytoplasm of the egg cell, allowing three natural barriers to be overcome outside the body. If the loss of highly valuable genetic material
 in a male or female parent is threatened, this may be the only way to preserve valuable hereditary traits with a high cost recovery, and it may also serve as the basis for further development of genetic engineering. In this regard, the Laboratory for Molecular Biotechnology and DNA Testing of the RUE “Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding” has developed and studied conditions for preparation of cattle oocytes for in vitro micromanipulation. It has been established that it is preferable to use the ovaries in the luteal and follicular phases.
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