Abstract
Ts 75 and ts 149 are two temperature-sensitive mutants of avian sarcoma virus B77 which fail to reproduce and to induce neoplastic transformation at 41°. Both mutants are indistinguishable from wild type in somatic properties of the virion: host range, type-specific antigenicity, and rate of inactivation at 41°. The temperature-sensitive step of ts 75 occurs late in the infectious cycle allowing the synthesis of group-specific antigen in increased amounts and of viral RNA under nonpermissive conditions. Ts 149 has an early temperature sensitive phase and does not produce group-specific antigen at 41°. The maintenance of some neoplastic properties in cells transformed by either ts 75 or ts 149 is continuously dependent on a temperature sensitive viral function: Shift of transformed cells to 41° results in disappearance of neoplastic traits. Double infection of cells at 41° with wild-type avian sarcoma virus and ts 75 or ts 149 results in the rescue of 3 markers derived from the mutant virus: temperature sensitivity, host range, and morphology of the transformed cell. Rescue of ts 75 or ts 149 with wild-type avian leukosis viruses at 41° has not been accomplished.
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