Condition‐dependent adenosine monophosphate decomposition pathways in striated adductor muscle from Japanese scallop (Patinopecten yessoensis)

Abstract

The purpose of this study was to confirm inosine monophosphate (IMP) generation and to clarify the decomposition pathway of adenosine monophosphate (AMP) by investigating the properties of AMP, IMP, and adenosine (AdR) decomposition enzymes in Japanese scallop (Patinopecten yessoensis). The results showed that IMP accumulated due to AMP decomposition via endogenous enzymes in scallops stored at both 4 °C and 20 °C. The AMP decomposition rate was highest in the supernatant of homogenized scallop adductor muscle, followed by the suspended solution and precipitate, while IMP could not be decomposed in scallop. The results indicated that the activity of AdR deaminase was very high, and this enzyme was involved in an intracellular process in scallop. Moreover, 1 min of heating exerted little influence on the AMP and AdR decomposition rates, while 5min of heating induced enzyme denaturation. The IMP generation rate increased dramatically in scallop crude enzyme solution containing 5mM ethylenediaminetetraacetic acid (EDTA). This suggests that the major pathway of AMP decomposition might change with variations in metal ion concentrations in Japanese scallop. PRACTICAL APPLICATION: IMP generation in Japanese scallop (Patinopecten yessoensis) caused by endogenous enzymes was confirmed. IMP is very important for the umami taste (a pleasant savory taste) of aquatic products. As IMP accumulation might be achieved by changing the concentration of divalent metal ions and no IMP 5'-nucleotidase activity was detected in scallop, a suitable process to produce good flavor scallops with high IMP contents might be developed.