Abstract
Bluetongue (BT) and peste-des-petits-ruminants (PPR) are major transboundary diseases of small ruminant, which are endemic in India. Testing of bluetongue virus (BTV) and peste-des-petits-ruminants virus (PPRV) from recent outbreaks (2015-2016) in different regions of Haryana State of India revealed that 27.5% of the samples showed the presence of dual infection of BTV and PPRV. Analysis of Seg-2 of BTV (the serotype-determining protein) showed the presence of BTV-12w in several isolates. However, analysis of N gene fragment amplicons showed that viruses belong to lineage IV were most closely related to a pathogenic strain of PPRV from Delhi. This is the first report of co-circulation of PPRV lineage IV and bluetongue virus serotype 12 in the state.
Highlights
Gupta, Akhil; Lala Lajpat Rai University of Veterinary and Animal Sciences (LUVAS), Department of Veterinary Microbiology
The Bluetongue virus (BTV) isolates were serotyped using quantitative real-time reverse transcription PCR (qRT-PCR) assays either using a panel of type specific qRT-PCR assays targeting Seg-2 (Maan et al, 2016), which revealed the presence of BTV-12 in all of isolates from different regions of Haryana showing concurrent infection of BTV and peste-despetits-ruminants virus (PPRV)
Details of primers used for amplification of N gene of Peste-des-petits-ruminants virus (PPRV) and VP2 gene of Bluetongue virus (BTV) for use in RT-PCR assays
Summary
Seg-2 of IND2015/340 showed 97.1% nt sequence identity with the reference strain of BTV-12 from South Africa Seg-2 of IND2015/340 showed greater variation (87.6% nt identity) from BTV-12 strain from The phylogeny inferred for BTV Seg-2 and PPRV N gene with the distance methods were consistent with those of the character-based analysis.
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