Concretionary methane-seep carbonates and associated microbial communities in Black Sea sediments

J Reitner,J Peckmann,M Blumenberg,W Michaelis,A Reimer,V Thiel

doi:10.1016/j.palaeo.2005.04.033

Abstract

Gas seeps in the euxinic northwestern Black Sea provide an excellent opportunity to study anaerobic, methane-based ecosystems with minimum interference from oxygen-dependent processes. An integrated approach using fluorescence- and electron microscopy, fluorescence in situ hybridization, lipid biomarkers, stable isotopes (δ13C), and petrography revealed insight into the anatomy of concretionary methane-derived carbonates currently forming within the sediment around seeps. Some of the carbonate concretions have been found to be surrounded by microbial mats. The mats harbour colonies of sulphate-reducing bacteria (DSS-group), and archaea (ANME-1), putative players in the anaerobic oxidation of methane. Isotopically-depleted lipid biomarkers indicate an uptake of methane carbon into the biomass of the mat biota. Microbial metabolism sustains the precipitation of concretionary carbonates, significantly depleted in 13C. The concretions consist of rectangularly orientated, rod- to dumbbell-shaped crystal aggregates made of fibrous high Mg-calcite. The sulphate-reducing bacteria exhibit intracellular storage inclusions, and magnetosomes with greigite (Fe3S4), indicating that iron cycling is involved in the metabolism of the microbial population. Transfer of Fe3+ into the cells is apparently mediated by abundant extracellular vesicles resembling known bacterial siderophore vesicles (marinobactine) in size (20 to 100 nm) and structure.

Highlights

Most methane seeping upward in the marine sedimentary column is intercepted biologically by a process known as anaerobic oxidation of methane (AOM; see Valentine, 2002 and Hinrichs and Boetius, 2003 for recent reviews)
Close to the Dniepr Canyon, in anoxic waters at depths between 200 and 400 m, methane seepage sustains the formation of authigenic carbonates within the sediment
The automicritic carbonate shows d13C values below À 20x V-PDB (Fig. 2B), indicating that a significant portion of the carbonate carbon is derived from methane

Summary

Introduction

Most methane seeping upward in the marine sedimentary column is intercepted biologically by a process known as anaerobic oxidation of methane (AOM; see Valentine, 2002 and Hinrichs and Boetius, 2003 for recent reviews). 13C-depleted, methane-derived carbonates (d13C b À 20x) are typically found at the seep locations. At sites of intense gas discharge, these precipitates may grow into the anoxic water column, forming tower-like buildups up to several metres in height (Ivanov et al, 1991; Luth et al, 1999; Peckmann et al, 2001; Lein et al, 2002; Michaelis et al, 2002)

Methods

Results

Conclusion