Abstract
Abstract Samples were collected randomly from different individuals of East District of Sikkim. Nepali (N = 110). Bhutia ( N = 75) and Lepcha (N = 48) in form of either blood or buccal swab as per cooperation of participent. Genomic DNA was extracted by using standard phenol/chloroform procedure (2). Quantitation of DNA was carried out using the Quantiblot kit (PE Applied Biosystems) and subsequent PCR amplification was performed using the Powerplex™ 16 multiplex System (Promega Coip, Madison, U.S.A.) The products were detected on a 5% denaturing polyacrylamide sequencing gel using the ABI Prism™ 377 DNA Sequencer (PE Applied Biosystems) and genotype classification was made by comparison with allelic ladders provided with the Powerplex™ 16 System.
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