Abstract
Y-chromosome short tandem repeat (STR) analysis is an important tool in forensic casework, especially in sexual assault cases. As Y-STR genetic profiles might be shared and compared between different laboratories, states and even countries, concordance of allele calls among commercial kits is an important consideration. In this work, we investigated the concordance of allele calls in twelve Y-chromosome STR loci (DYS19, DYS385, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439) between the Investigator Argus Y-12 QS (Qiagen) and the AmpFlSTR YfilerTM (Life Technologies) kits in a sample of 155 male blood donors from BrasÃlia, Federal District, Brazil.
Highlights
Y-chromosome short tandem repeat (STR) analysis is an important tool in forensic casework, especially in sexual assault cases
We investigated the concordance of allele calls in twelve Y-chromosome STR loci (DYS19, DYS385, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439) between the Investigator Argus Y-12 QS kit (Qiagen) and YfilerTM (Life Technologies) in 155 previously typed samples
Samples were previously typed using the AmpFlSTR® YfilerTM PCR Amplification Kit (Life Technologies) [1] and in this work typed with the Investigator Argus Y-12 QS kit, according to the manufacturer’s instructions
Summary
Y-chromosome short tandem repeat (STR) analysis is an important tool in forensic casework, especially in sexual assault cases. As Y-STR genetic profiles might be shared and compared between different laboratories, states and even countries, concordance of allele calls among different commercial kits is an important consideration. We have previously typed 300 samples from male blood donors from Brasília, Federal District, Brazil with the AmpFlSTR YfilerTM kit (Life Technologies) [1].
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