Abstract
Xylocarpus granatum, a common mangrove plant is traditionally used for the treatment of diarrhoea, cholera, fever, dyslipidemia, inflammation, etc. The present study was carried out to evaluate the antioxidant and anti-inflammatory potential of the ethanolic extract of X. Granatum by various in vitro methods such as 1,1-diphenyl-2-picryl-hydrazil (DPPH) free radical scavenging assay, reducing power assay, ferric reducing antioxidant power (FRAP) and β-carotene bleaching inhibition assay. Total phenolic and flavonoid content were determined. Anti-inflammatory activity was evaluated by in vitro human RBC membrane stabilizing assay and in vivo mice paw edema test. Ethanolic leaf extract (S2) of X. granatum showed significant scavenging effect on DPPH scavenging with a value of IC50 165.95µg/ml. In addition, it showed significant reducing potential with a value of 59.04 mM of ferrous equivalent per ml in FRAP assay and in reducing power assay the EC50 value was determined as 241.61μg/ml . The ethanolic leaf extracts exhibited 72.3% β-carotene bleaching inhibition. The total phenolic and flavonoid content of the extract were 66μg/ml gallic acid equivalent and 47.66μg/ml quercetin equivalent per gram of dry extract, respectively. The extract also exhibited 52.63% and 51.05% protection of RBC membrane in hypotonicity and heat induced lysis inhibition, respectively. Significant reduction of mice paw edema (36.34% in 20 μg/kg bw concentration) was observed in the extract. The results revealed that the leaf extract of X. granatum possesses strong antioxidant and anti-inflammatory potential.
 
 J Bangladesh Agril Univ 17(4): 466–475, 2019
Highlights
Metabolism implies oxidative processes which are vital for cell proliferation and survival
The results revealed that the leaf extract of X. granatum possesses strong antioxidant and anti-inflammatory potential
Antioxidant, the plant secondary metabolites are mostly phenolic compounds which play vital role in neutralizing free radicals of reactive oxygen species (ROS) by donation of electron or by inhibition of enzymes involved in oxidation and inflammation
Summary
Metabolism implies oxidative processes which are vital for cell proliferation and survival. ROS play an important role in numerous physiological and pathological processes including inflammatory diseases (Droge, 2002). High level of ROS is involved in carcinogenesis and other diseases related to oxidative damage. Appropriate level of ROS is outmost necessity for cell proliferation, survival, growth and apoptosis. Maintaining the appropriate level of ROS is essential for healthy condition where antioxidant play crucial role through neutralizing ROS. The increment of intake of exogenous antioxidant would ameliorate the damage caused by oxidative stress. The demand of various antioxidants is increasing in food industries for functional food and fortification of processed food products. Antioxidants are very useful active ingredients for the manufacturing of cosmetics including dermatological protection of skin from photo damage and aging
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
