Abstract
Objective: ?-carrageenan is a food stabilizer agent which has an antiproliferative effect, while vitamin D is a prohormone acts on the nuclear receptor and has a cytotoxic against cancer. This study aimed to show the synergistic effect of using topical ?-carrageenan and oral administration of the vitamin D on the 7, 12-dimethylbenz[a] anthracene (DMBA)-induced oral cancer. Material and Methods: fifty four male albino rats were randomly divided into seven groups: Acetone-treated served as control (Group I), vitamin D (5000UI)-treated (Group II), ?-carrageenan (1%)- treated (Group III), DMBA (0.5%)-treated (Group IV), Acetone, ?-carrageenan and DMBA were administered topically on both cheeks and palate, five times weekly for 12 weeks, while the vitamin D was administered orally twice weekly for 12 weeks. Groups V, VI, and VII were animals treated with vitamin D, ?-carrageenan, and both vitamin D and ?-carrageenan for 8 weeks after induction of oral cancer. At the end of the study, blood samples were obtained by cardiac puncture for determination of TNF-? and EGFR. Results: In the groups III and IV, serum EGFR showed significant low levels compared with Group I. In the Group VII, serum EGFR showed a significantly (p=0.014) low level compared with Group IV (614.3±69.7 pg/ml versus 882.4±45.6 pg/ml, respectively). Higher percentages of high levels of TNF-? were observed in the Groups VI and VII, while a lower percentage of EGFR was observed in the Group VI. Conclusion: both ?-carrageenan and vitamin D have antiproliferative effect against DMBA-inducing oral cancer by increasing the levels of TNF-? and suppressing the signaling pathway of EGFR. Concomitant using ?-carrageenan and vitamin D reduces the antiproliferative effect of each other. KeywordsOral cancer; 7, 12-dimethylbenz[a] anthracene; Vitamin D; ?-carrageenan; Epidermal growth factor receptor; Tumor necrosis factor-?.
Highlights
Animals treated with κ-carrageenan (Group III) showed a significantly lower serum level of epidermal growth factor receptor (EGFR) compared with the corresponding level of Group I, which account a 22.2% decrease
Animals treated with simultaneous administration of an oral dosage of vitamin D and topical application of κ-carrageenan (Group VII) showed a significantly lower serum level of EGFR compared with the corresponding level of Group I (614.3±69.7pg/ml versus 1118.5±91.8 pg/ml, p=0.014), which accounts 45.1% decrease)
Oral administration of vitamin D (Group V) and topical application of κ-carrageenan (Group VI) produced a non-significant decrease of the EGFR serum levels in rats treated with topical application of DMBA by 8.9% and 13.6% of the serum level of EGFR of rats treated with DMBA alone (Group IV)
Summary
Groups V, VI, and VII were animals treated with vitamin D, κ-carrageenan, and both vitamin D and κ-carrageenan for 8 weeks after induction of oral cancer. Os grupos V, VI e VII foram animais tratados com vitamina D, κ-carragenina e No final do estudo, foram obtidas amostras de sangue por punção cardíaca para determinação do TNF-α e EGFR. Resultados: Nos grupos III e IV, o EGFR sérico mostrou níveis baixos significativos em comparação com o Grupo I. No grupo VII, o EGFR sérico mostrou um nível significativamente baixo (p = 0,014) em comparação com o Grupo IV (614,3 ± 69,7 pg / ml versus 882,4 ± 45,6 pg / ml, respectivamente). Concomitant using of topical carrageenan-kappa and oral vitamin D against 7, 12-dimethylbenz[a] anthracene induced-oral cancer in rats: A synergism or an antagonism effects
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
