Concomitant Detection of Biofilm Formation and MBL Production in Meropenem Resistant Isolates of Pseudomonas aeruginosa

Abstract

The purpose of this study was to detect biofilm formation and to examine the correlation between biofilm and Metallo-β-lactamases (MBL) production in Pseudomonas aeruginosa. A total of 64 P. aeruginosa isolates were identified using standard microbiological methods and antimicrobial susceptibility testing (AST) was performed on them according to Clinical and Laboratory Standards Institute (CLSI) guidelines. The isolates were screened for biofilm production using both qualitative and quantitative methods. The presence of MBL genes were checked by multiplex PCR assay. Out of all 30 meropenem resistant P. aeruginosa , 2 isolates were found producing all the three genes (i.e. bla IMP , bla VIM , bla SIM ) for MBL production and they were found to produce biofilm. Resistant to four antibiotics such as aztreonam (85.7% vs 11.1%, P< 0.000), Cefepime (82.1% vs 2.8%, P<0.000) gentamycin (82.1% vs 27.8%, P< 0.000) and Pipercillin/Tazobactum was also high