Abstract
AbstractA library of phosphoramidite monomers containing a mainâchain cleavable alkoxyamine and a sideâchain substituent of variable molar mass (i.e. mass tag) was prepared in this work. These monomers can be used in automated solidâphase phosphoramidite chemistry and therefore incorporated periodically as spacers inside digitallyâencoded poly(phosphodiester) chains. Consequently, the formed polymers contain tagged cleavable sites that guide their fragmentation in mass spectrometry sequencing and enhance their digital readability. The spacers were all prepared via a seven steps synthetic procedure. They were afterwards tested for the synthesis and sequencing of model digital polymers. Uniform digitallyâencoded polymers were obtained as major species in all cases, even though some minor defects were sometimes detected. Furthermore, the polymers were decoded in pseudoâMS3 conditions, thus confirming the reliability and versatility of the spacers library.
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