Abstract
The article presents the results of the research on the cultivation of B. bifidum and B. subtillis in a high-carbohydrate nutrient medium composed of beet molasses, obtaining metabolites and scientific and practical substantiation of the resulting culture liquid as a biologically active additive. The material of the laboratory studies was sugar beet molasses of 5%-10%-15%-20%-25% concentrations. The studies used the probiotic microorganism B. bifidum, strain No. 1 and B. subtilis, strain DSM-3242. To obtain bacterial concentrate, a daily culture of microorganisms was used, which was standardized to 1×106 CFU / cm3. The testing process was carried out according to the indicators of the mass fraction of fermented sugars, determined by the calculation method, branch standard OCT 18-395-82. The number of CFU was calculated in the Goryaev chamber using the method of GPhA (General Pharmacopeic Article) 1.7.2.0008.15. The content of amino acids, vitamins, and organic acids in the culture fluid was determined by capillary electrophoresis in the device “Drops”. During the cultivation of B. bifidum and B. subtillis, a certain metabolic activity of their vitality was found, which is expressed in a decrease in the mass fraction of the amount of fermented sugars in the range of 53.7% to 23.7%. The hydrogen index is from 6.70 to 3.77 in experimental samples of molasses. The number of B. bifidum and B. subtillis CFUs cultivated on 5% molasses on the second day reached 7x106 CFU/cm3 and 8.2 × 106 cm3. In other concentrations, a maximum indicator of the number of microorganisms occurred on the third day and did not exceed 6.6 and 7 × 106 CFU/cm3. A significant synthesis of metabolites in the form of indispensable and conditionally dispensable amino acids and organic acids was determined. The dynamics of amino acid synthesis is consistent with the amylolytic and enzymatic activity of microorganisms in the molasses culture medium. In this case the level of enzymes is directly dependent on the number of CFU.
Highlights
At present the Russian market of probiotics requires combined preparations that are part of a complex probiotic in which bacterial strains are united by their capability to produce different enzymes, biologically valuable substances in such a way that they will add each other by biological activity
In the studies [4,5,6] the improvement of these preparations is carried out in the following directions: search for new highly active strains of bifidobacteria and lactic acid bacteria; development of probiotics based on associated microbial cultures; use of auto- and donor strains of endogenous microorganisms for the design of new preparations
They participate in enzymatic processes, perform a vitamin-forming function, improve the indicators of protein, lipid and mineral metabolism, as they enhance the hydrolysis of proteins, ferment carbohydrates, saponify fats, dissolve fiber
Summary
At present the Russian market of probiotics requires combined preparations that are part of a complex probiotic in which bacterial strains are united by their capability to produce different enzymes, biologically valuable substances in such a way that they will add each other by biological activity. B. bifidum is a species of Gram-positive anaerobic bacteria They participate in enzymatic processes, perform a vitamin-forming function (synthesis of B vitamins, vitamin K, folic and nicotinic acids), improve the indicators of protein, lipid and mineral metabolism, as they enhance the hydrolysis of proteins, ferment carbohydrates, saponify fats, dissolve fiber. They stimulate intestinal peristalsis, promote normal intestinal cleansing, promote the synthesis of essential amino acids, better absorption of calcium salts, vitamin D, have anti-anemic, antirachitic and anti-allergic effects, stimulate the lymphoid apparatus. The beneficial properties of bifidobacteria include the ability to effectively absorb lactose, stimulate the immune system, reduce cholesterol in the blood, and have an anticarcinogenic effect [6,8,9]
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