Abstract
Incorporation of 2-deoxy-D-glucose into cultured rhesus diploid cells includes transport and subsequent phosphorylation with resultant accumulation of both free and phosphorylated sugar. Accumulation of the free sugar proceeds to a maximal limit of 4–5 mM which is determined by intrinsic cell factors and is independent of medium 2-deoxy-D-glucose up to 5 mM concentration. Concentrative accumulation (active transport) pf the free sugar is readily demonstrable on maintaining medium concentrations of 2-deoxy-D-glucose at less than the maximal accumulation potential of the cells (i.e. <4–5 mM). Cellular concentrations of the free sugar in excess of 30-times medium concentrations are demonstrable on incubation of the cells in the presence of <0.05 mM 2-deoxy-D-glucose. In contrast, accumulation of 2-deoxy-D-glycose is not demonstrable in the human erythrocyte.
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