Concentrations of Interleukin-1ß in Gingival Crevicular Fluid and Saliva - a Potential Diagnostic Biomarker of Periodontal Diseases.

Theodora Bolyarova-Konova,Ekaterina Ivanova-Todorova,Hristina Mihaylova,Dolores Todorova,Kalina Tumangelova-Yuzeir,Silviya Petkova,Tsvetelina Velikova

doi:10.3897/folmed.62.e49872

Theodora Bolyarova-Konova, Ekaterina Ivanova-Todorova + Show 5 more

Open Access

https://doi.org/10.3897/folmed.62.e49872

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Journal: Folia medica	Publication Date: Dec 31, 2020
Citations: 2	License type: CC BY 4.0

Affiliation: Medical University of Sofia

Abstract

Bacterial challenge in periodontal diseases activates both local and systemic immune responses of a macroorganism by increasing multiple proinflammatory factors that can be discovered in gingival crevicular fluid (GCF) and in saliva. We tested the hypothesis that IL-1β concentration in GCF and saliva correlates with periodontal health and diseases.Materials and methods: The study included 62 people (mean age 36±14 yrs), divided into three groups - patients with periodontitis (24 people), patients with gingivitis (19 people) and periodontally healthy people (19 people). Saliva and GCF samples were taken from all participants and the levels of IL-1β in all samples were determined by ELISA. IL-1β concentrations in GCF of healthy individuals were significantly lower than the IL-1β concentration in GCF of patients with gingivitis (p=0.009) and with periodontitis (p.

Highlights

Bacterial challenge in periodontal diseases activates both local and systemic immune responses of a macroorganism by increasing multiple proinflammatory factors that can be discovered in gingival crevicular fluid (GCF) and in saliva
The IL-1β concentration in GCF in group of healthy individuals were significantly lower than the IL-1β concentration in GCF in group of patients with gingivitis (Mann-Whitney test, p=0.009)
The IL-1β concentrations in the GCF in group of healthy individuals were significantly lower than the IL-1β concentrations in GCF in group of patients with periodontitis (Mann-Whitney, test p

Summary

MATERIALS AND METHODS

Periodontitis is a chronic multifactorial inflammatory disease associated with dysbiotic plaque biofilms and characterized by progressive destruction of the tooth-supporting apparatus. The conventional methods for periodontal diagnosis most often include measuring of pocket depths, clinical attachment loss, bleeding on probing and x-ray findings of bone loss. These data reflect the severity of the periodontitis and the changes which were caused as a result of the disease’s development in the past; they cannot rate the disease’s activity at the moment of examination.[6]. We tested the hypothesis that IL1β in GCF and IL-1β in saliva correlates with periodontal state – health, gingivitis, and periodontitis.

RESULTS

DISCUSSION

CONCLUSIONS