Concentrations of a PGF2α metabolite during pregnancy on the days that luteolysis occurs in nonbred heifers

Abstract

Concentrations of a metabolite of PGF2α (PGFM) were compared between nonbred (n = 6) and pregnant (n = 8) heifers on days 16, 17, and 18 postovulation. On each day, an 8-h session of hourly blood sampling was done. Averaged over the 8-h sessions, mean concentration of PGFM was less (P < 0.0009) in the pregnant group (45.2 ± 3.2 pg/mL) than that in the nonbred group (65.6 ± 7.9 pg/mL), but the minimal concentration per session was not significantly different between groups. Pulses of PGFM (identified by coefficient of variance) were similar in frequency between groups but were less (P < 0.03) prominent at the peak in the pregnant group (60.0 ± 5.3 pg/mL) than that in the nonbred group (92.8 ± 10.7 pg/mL). These results indicated similarity between groups in frequency and initial development of a PGFM pulse but without later development and a reduction in prominence in the pregnant group. The progesterone response to a PGFM pulse of similar prominence was made before the beginning of luteolysis in individuals in the nonbred group and during the hourly sessions on days 16 to 18 in the pregnant group. Progesterone concentration in the nonbred group decreased (P < 0.05) during 2 h before the PGFM peak (8.8 ± 1.6 to 5.6 ± 1.0 ng/mL) and rebounded (P < 0.05) completely during the 2 h after the peak (5.6 ± 1.0 to 9.6 ± 2.2 ng/mL). A transient progesterone decrease during a similar PGFM pulse and similar initial progesterone concentration did not occur in the pregnant group. Results supported the hypotheses that (1) pregnant heifers have identifiable but less prominent PGFM pulses during the days that luteolysis occurs in nonbred heifers and (2) the corpus luteum locally resists the luteolytic effect of PGF2α in pregnant heifers before the days of onset of luteolysis in nonbred heifers.