Concentration of soybean lecithin affects short-term storage success of goat semen related with seminal plasma removal.

Robespierre Augusto Joaquim Araújo Silva,Lúcia Cristina Pereira Arruda,Maria Madalena Pessoa Guerra,Sildivane Valcácia Silva,André Mariano Batista,Wilton Arruda Gomes,Helder Melo De Souza,Igor Henrique De Azevedo Valença Nery,Pierre De Castro Soares

doi:10.21451/1984-3143-ar2019-0012

Robespierre Augusto Joaquim Araújo Silva, Lúcia Cristina Pereira Arruda + Show 7 more

Open Access

https://doi.org/10.21451/1984-3143-ar2019-0012

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Abstract

The objective of this study was to investigate the need of seminal plasma removal for short-term cooling of buck semen in soybean lecithin (SL) based extender. Each pool was divided equally, and one half was subjected to centrifugation to remove seminal plasma (SP-), while the other half remained with seminal plasma (SP+). Then, both SP+ and SP- samples were diluted in two SL extenders (extender A = 1% SL; extender B = 2% SL), cooled to 5ºC and stored for 48 hours. The sperm kinetics, evaluated by CASA, and plasma membrane integrity (PMI), acrosomal integrity (ACI) and high mitochondrial membrane potential (HMMP), evaluated by epifluorescence microscopy, were determined within five minutes after reaching 5°C (T0), as well as after 24 (T24) and 48 (T48) hours of storage. Interactions (seminal plasma vs. extender vs. time;) were observed for all variables assessed. Total and progressive motility and other variables of sperm kinetics decreased after 24 hours of cooling in the SP+ group, and after 48 hours of storage, these same variables were lower in SP+/B compared to SP-/B groups. Furthermore, SP+ reduced PMI (extender B, T48), HMMP (A and B extenders, T48) and ACI (extender A, T0) compared to SP- samples. The interactions between seminal plasma and soybean lecithin phospholipids seemed to occur in a time-dependent manner. It was concluded that the removal of seminal plasma improves the quality of goat semen that was cooled in a soybean lecithin-based extender, especially when using 2% soybean lecithin.

Highlights

Liquid-stored semen can be an alternative to frozen-thawed semen for artificial insemination, since semen cryopreservation is an expensive process (Liu et al, 2016)
Significant interactions were observed between seminal plasma (SP), levels of soybean lecithin (E) and cooling time (T) for all variables analyzed in this study
In samples maintained with seminal plasma (SP+), sperm kinetics (TM, progressive motility (PM), LIN, STR, WOB, VSL and VAP)

Summary

Introduction

Liquid-stored semen can be an alternative to frozen-thawed semen for artificial insemination, since semen cryopreservation is an expensive process (Liu et al, 2016). Much research has already been conducted to prolong the in vitro viability and fertilizing potential of stored liquid semen, limited improvements have been. Traditional extenders for goat semen cryopreservation include egg yolk, skimmed milk or their combination (Purdy, 2006). Enzymes in the seminal plasma of bucks may interact with specific components of skimmed milk or egg yolk, rendering extenders containing these substances harmful to the bucks’ spermatozoa (Pellicer-Rubio et al, 1997; Leboeuf et al, 2000; Aboagla and Terada, 2004). The SL contains a mixture of phospholipids, fatty acids and low-density lipoproteins, which protects the sperm cell membranes by restoring the phospholipids lost during heat shock (Forouzanfar et al, 2010; Oke et al, 2010)

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