Concentration of recombinant baculovirus by cation-exchange chromatography.

Abstract

BioTechniquesVol. 26, No. 5 BenchmarksOpen AccessConcentration of Recombinant Baculovirus by Cation-Exchange ChromatographyJames BarsoumJames Barsoum*Address correspondence to Dr. James Barsoum, Biogen, Inc., 12 Cambridge Center, Cambridge, MA 02142, USA. Internet: E-mail Address: jim_barsoum@biogen.comBiogen, Cambridge, MA, USASearch for more papers by this authorPublished Online:22 Aug 2018https://doi.org/10.2144/99265bm07AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInRedditEmail FiguresReferencesRelatedDetailsCited ByProduction of Baculovirus and Stem Cells for Baculovirus-Mediated Gene Transfer into Human Mesenchymal Stem Cells22 September 2020Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus SystemExpression and purification of recombinant feline interferon in the baculovirus-insect larvae systemProcess Biochemistry, Vol. 49, No. 6Virus Separation Using Membranes20 November 2013Membrane Supported Virus Separation from Biological Solutions14 June 2013 | Chemie Ingenieur Technik, Vol. 85, No. 8Purification of a recombinant baculovirus of Autographa californica M nucleopolyhedrovirus by ion exchange membrane chromatographyJournal of Virological Methods, Vol. 183, No. 2Rational design and optimization of downstream processes of virus particles for biopharmaceutical applications: Current advancesBiotechnology Advances, Vol. 29, No. 6Nonintegrating DNA Virus25 October 2011Baculovirus as vectors for human cells and applications in organ transplantationJournal of Invertebrate Pathology, Vol. 107Quality control and analytical methods for baculovirus-based productsJournal of Invertebrate Pathology, Vol. 107Requirements for baculoviruses for clinical gene therapy applicationsJournal of Invertebrate Pathology, Vol. 107Development and validation of a HPLC method for the quantification of baculovirus particlesJournal of Chromatography B, Vol. 879, No. 1High-titer preparation of Bombyx mori nucleopolyhedrovirus (BmNPV) displaying recombinant protein in silkworm larvae by size exclusion chromatography and its characterization12 June 2009 | BMC Biotechnology, Vol. 9, No. 1Enhancing the multiplication of nucleopolyhedrovirus in vitro by manipulation of the pHJournal of Virological Methods, Vol. 161, No. 2Purification of recombinant baculoviruses for gene therapy using membrane processes2 April 2009 | Gene Therapy, Vol. 16, No. 6Concanavalin a affinity chromatography for efficient baculovirus purificationBiotechnology Progress, Vol. 23Virus Production for Clinical Gene Therapy24 February 2009Targeting and Purification of Metabolically Biotinylated BaculovirusHuman Gene Therapy, Vol. 19, No. 6Baculovirus-Infected Insect Cells Expressing Peptide-MHC Complexes Elicit Protective Antitumor Immunity20 December 2007 | The Journal of Immunology, Vol. 180, No. 1Baculovirus envelope fusion proteins F and GP64 exploit distinct receptors to gain entry into cultured insect cellsJournal of General Virology, Vol. 88, No. 12Ion-Exchange Membrane Chromatography Method for Rapid and Efficient Purification of Recombinant Baculovirus and Baculovirus gp64 ProteinHuman Gene Therapy, Vol. 18, No. 7High yield purification of functional baculovirus vectors by size exclusion chromatographyJournal of Virological Methods, Vol. 142, No. 1-2Sampling Viruses from Soil14 May 2007Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus SystemBaculovirus Vectors for Gene TherapyBaculovirus as a highly efficient expression vector in insect and mammalian cellsActa Pharmacologica Sinica, Vol. 26, No. 4Highly efficient baculovirus-mediated gene transfer into rat chondrocytes1 January 2004 | Biotechnology and Bioengineering, Vol. 88, No. 5Generation of chimeric baculovirus with histidine-tags displayed on the envelope and its purification using immobilized metal affinity chromatographyEnzyme and Microbial Technology, Vol. 33, No. 4Baculovirus as Mammalian Cell Expression Vector for Gene Therapy: An Emerging StrategyMolecular Therapy, Vol. 6, No. 1A novel system for the production of fully deleted adenovirus vectors that does not require helper adenovirus22 June 2001 | Gene Therapy, Vol. 8, No. 11Estimation of Baculovirus Titer by β-Galactosidase Activity Assay of Virus PreparationsTetsuro Yahata, Stephanie Andriole, Kurt J. Isselbacher & Toshi Shioda29 August 2018 | BioTechniques, Vol. 29, No. 2Application of Recombinant Baculoviruses in Biopharmaceutical Research Vol. 26, No. 5 Follow us on social media for the latest updates Metrics Downloaded 370 times History Published online 22 August 2018 Published in print May 1999 Information© 2018 Author(s)PDF download