Abstract
A method for the further purification of a fraction salted-out from a dilute hot hydrochloric acid extract of untreated ground bovine parathyroid glands is described. Chromatography on carboxymethylcellulose using a NaCl gradient rising to I M at pH 4.68 results in an active Ca-mobilizing fraction with a 6-fold increase in specific activity in moderate yield. This can be further increased 2- to 6-fold by recycling and eluting with a gradient rising to 0.5 M salt. The method is applicable to starting material containing either high or low amounts of activity.
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