Abstract

Moon phase is one of the external factors that affect the dynamics of crab moulting hormones (ecdysteroids). This study aimed to determine the effect of moon phase on the moulting hormone concentration of the mud crab Scylla olivacea Herbst 1796. Data were collected on the haemolymph ecdysteroid concentration in the eight phases of the moon: new moon, 1/4 I, 1/2 I, 3/4 I, full moon, 3/4 II, 1/2 II and 1/4 II. Ultra-Fast Liquid Chromatography (UFLC) was applied for haemolymph moulting hormone analysis. The results showed that the pattern of haemolymph ecdysteroid concentration varied based on the phases of the moon. The highest haemolymph ecdysteroid concentration was found in 1/2 I and 1/2 II moon phases. During this phase the moon exerts a relatively weak gravitational force. The lowest haemolymph ecdysteroid concentration was found in the new and full moon phases during which the moons gravitational pull is the strongest.

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