Concentration of Lissamine green in proximal tubules of antidiuretic and mercury poisoned rats and the permeability of these tubules.

Abstract

By coloring castor oil with Sudan-red we were able to modify the split droplet method so that concentrations of Lissamine green (LG) could be determined by microphotometry in individual proximal tubular loops of rat kidneys. In the course of the split droplet experiments an increase in the concentration of LG occured, which corresponded to the decrease in volume of the solvent (calculated from the intratubular diameter and the length of the test droplet) only with test lengths of over 100 μ. The change in concentration at shorter lengths of test droplets may have resulted from an incomplete blockage between the oil block and the test droplet.