Concentration of isoprostanes as a marker reflecting oxidative status in moderate dyslipidemia

Abstract

Dyslipidemia and oxidative stress are considered one of the major causes which lead to many diseases, especially atherosclerosis development, cardiovascular disorders and aggravate risk for cancers, aging and others. Lipid hydroperoxides are the initial products when lipids are damaged by free radicals and in plasma HDL is their major carrier. F2-IsoP are stable compounds and specific products of lipid peroxidation only in phospholipids in vivo through nonenzymatic free-radical mechanisms. Also, they are found in detectable quantities esterified in all biological tissues and in free form in all normal biological fluids. Quantifying of them is considered as accurate way to measure oxidative stress in vivo and could be used as an indicator of lipid peroxidation which process is a risk factor for atherosclerosis and other systemic diseases. Our study was performed in the group of young people (n=10, age 19-30) with moderate dyslipidemia and the reference group. In both groups we have investigated serum lipids (TG, TC, LDL-C, HDL-C, nonHDL-C), lipoproteins (apoAI, apoB) and lipid (TC/HDL-C, TG/HDL-C, LDL-C/HDL-C) and lipoprotein (HDL-C/apoAI) ratios and oxidative status by measuring free and total (free and esterified) F2-isoprostanes (F2-IsoP). According to the available data reports and taking into account results obtained in our study we concluded that even mild lipid and lipoprotein abnormalities which are often present in population might by connected with elevated oxidative status which is sensitively reflected by F2-IsoP concentration. Thus measurement of F2-IsoP seems to be very helpful in recognition of early stage of oxidative stress however further studies are required.