Abstract
Immunreactive FSH, LH and Prolactin are measured in seminal fluid and serum following i.v. injection of LH-RH and TRH. Levels of hormone levels in serum and seminal fluid are not correlated. Only LH levels, but not those of FSH and PRL depend on the interval between injection of releasing factors and ejaculation. Dilution curves and column chromatography of seminal fluid LH point that it might not be identical to serum LH. Since in the testis a HCG immunreactive substance has been described it is likely that it forms part of the LH concentrations measured in seminal fluid.
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