Abstract
The aim of this study was to determine apolipoprotein (apo) E concentration in the high-density lipoprotein (HDL) fractions of normolipidemic subjects. ApoE concentrations in total blood plasma and HDL fractions were measured by an immunoturbidimetric method. We observed that the quantitative distribution of apoE among different lipoprotein classes depends on the total plasma apoE concentration: at low total plasma apoE concentration, a substantial amount of apoE was associated with HDL; an increase in total plasma apoE was accompanied by a more equal distribution of apoE among lipoprotein fractions. The concentration of apoE in the HDL fraction was stable and did not depend on the total plasma apoE concentration. Thus, the preservation of a constant concentration of apoE in HDL due to its redistribution among lipoprotein classes is a priority when total plasma apoE concentrations change. This feature should be considered at diagnosis and for the treatment of lipid disorders.
Highlights
ApoE is a monomeric glycoprotein with 299 amino acid residues and a 34 kDa molecular mass (Rall et al, 1982)
It is believed that the change in apoprotein content of lipoproteins plays a key role in the initiation of many physiologically important metabolic processes
The majority of apoE in the plasma is associated with high-density lipoprotein (HDL) (Blum et al, 1980; Phillips et al, 1983; Fredenrich et al, 1997)
Summary
ApoE is a monomeric glycoprotein with 299 amino acid residues and a 34 kDa molecular mass (Rall et al, 1982). ApoE participates in the synthesis of some lipoproteins, in the transport and redistribution of lipids among various tissues including the cholesterol transport from peripheral tissues to the liver and in the repair of nervous cells and the vascular wall This protein has been shown in vitro to have antioxidant and anti-inflammatory properties (Eisenberg, 1984; Koo et al, 1985; Mahley, 1988; Hayek et al, 1994; Kelly et al, 1994; Vogel et al, 1994; Mabile et al, 2003; Ali et al, 2005; Pham et al, 2005; Boiko and Kaneva, 2009)
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