Abstract
In this study, electrowetting on dielectric (EWOD) was applied to a droplet of particle or algal solution to [1] suppress the coffee ring effect occurred during evaporation process, [2] concentrate the particle or algal cells and [3] shorten the processing time for analysis. The results showed that the micro particles and algal cells were effectively concentrated within approximately 15 minutes on the Teflon-coated microchip with interdigitated electrodes operated at the applied frequencies around 1k Hz and voltage 300 Vpp. The evaporation time for the sessile drop decreased substantially when applying EWOD and the size of the spot could be reduced to approximately 16% of that of the initial droplet. For algal solution with OD682 value equal to 5.32, strong Raman signals of microalgae were obtained near the central region of the dried algal spot.
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