Concentration of ω‐3 polyunsaturated fatty acids of marine oils using Candida cylindracea lipase: Optimization of reaction conditions

Abstract

AbstractProduction of ω‐3 fatty acid concentrates from seal blubber oil (SBO) and menhaden oil (MHO) upon enzymatic hydrolysis by Candida cylindracea lipase was optimized. In this process, the content of total ω‐3 fatty acids, Y1; eicosapentaenoic acid, Y2; and docosahexaenoic acid, Y3, in the final product was maximized. A three‐factor central composite rotatable design was used to study the effect of enzyme concentration (X1), reaction time (X2), and reaction temperature (X3). Second‐order polynomial regression models for Y1, Y2, and Y3 were employed to generate response surfaces. After hydrolysis, a maximum of 54.3% total ω‐3 fatty acids was obtained from SBO at an enzyme concentration of 308 U/g oil, a reaction time of 40 h, and a reaction temperature of 37°C. Similarly, a maximum of 54.5% total ω‐3 fatty acids was obtained from MHO at an enzyme concentration of 340 U/g oil, a reaction time of 45 h, and a reaction temperature of 38°C.