Abstract
The quantity of sorbic and benzoic acids in soft drinks is determined by reverse‐phase high performance liquid chromatography (HPLC) method. A good separation of the two preservatives was obtained by using a mobile phase composed of ammonic acetate 5 mM at pH 4.4 and aceto‐nitrile (80/20), and with UV detection. The average levels of benzoic acid varied between 73.4 ±2.51 (brand F) and 429 ±2.21 (brand K), and those of sorbic acid between 72.3 ±1.04 (brand M) and 199 ± 7.72 (brand A). Likewise, the estimated daily intake (EDI) for each additive was calculated, resulting in a higher intake of benzoic acid (38.3 mg) than sorbic acid (5.98 mg). In both cases, the values were lower than the acceptable daily intake (ADI).
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