Abstract
The development of an expanded bed process for the concentration and purification of orotic acid directly from whey is described. Different commercially available adsorbents were tested in series of pilot batch adsorption experiments to determine the most suitable separation system. Best results were achieved using a weak anion-exchange matrix. An elution protocol was established using MgCl 2 as eluting agent to recover the adsorbed orotic acid with ∼85% yield and 10-fold concentration. Purified orotic acid was precipitated under acid conditions with a yield of 95%.
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