Concentration and molecular weight distribution of hyaluronate in synovial fluid from clinically normal horses and horses with diseased joints

Abstract

Summary High molecular weight (mw) hyaluronate (ha) is an integral part of synovial fluid (sf), regulating many Important physiologic and pathophysiologic mechanisms. Many of its effects depend on, or are reflected in. the concentration and mw of ha. High-performance liquid chromatography was used to assess simultaneously the concentration and mw of ha in sf obtained from horses with various arthritides: acute traumatic arthritis; chronic traumatic arthritis, including degenerative joint disease (djd); and infectious arthritis. The size-exclusion column was calibrated, using appropriate ha concentration and mw standards, before the high-performance liquid chromatographic assays of the sf samples. Calibration of the column disclosed that the maximal limit for mw estimation of ha was around 3 million. In control joints, mw of ha ranged from 2 to 3 × 106 (mean 2.5 × 106) and did not differ significantly from mw of ha in sf from horses with acute or chronic traumatic arthritis (mean 2 × 106; range 1.5 to 3 × 106). Interestingly, a small amount of ha of moderately high mw (approx 1 to 1.5 × 106) was detected in chromatograms of sf from infected joints. This degree of polymerization of sf ha was significantly (P < 0.01) lower, compared with that for control joints. There was no difference in mean (± sd) concentration of ha between control joints and joints with acute or chronic traumatic arthritis (0.33 ± 0.12 g/L vs 0.18 ± 0.03 g/L. or 0.23 ± 0.12 g/L.), indicating that sf ha concentration probably should not be used as a diagnostic marker for the condition. However, the sf ha concentration was significantly (P < 0.01) lower in joints with infectious arthritis (0.07 ± 0.03 g/L) and in the joints with radiographic evidence of djd (0.12 ± 0.01 g/L), compared with control joints.