Concentration and cultivar effects on efficacy of CLO-1 biofungicide in controlling Fusarium head blight of wheat

Abstract

Fusarium head blight (FHB) is a destructive disease of wheat in Canada and Clonostachys rosea strain ACM941 has been identified as a promising biological control agent for managing FHB. In the present research the concentration and cultivar effects on the efficacy of CLO-1, a formulated product of C. rosea strain ACM941, in controlling FHB and deoxynivalenol (DON) contamination in wheat was studied. Of the eight concentrations ranging from 104 to 108cfumL−1 evaluated, significant effects were generally observed for concentrations at or above 106cfumL−1 in the greenhouse and field trials in 2009 and 2010. In the greenhouse, CLO-1 reduced the area under the disease progress curve (AUDPC) by 65–83%, Fusarium damaged kernels (FDK) by 68–92%, and DON by 51–95%. Under field conditions, CLO-1 reduced FHB index by 30–46%, FDK by 31–39%, and DON by 22–33%. These effects were numerically lower but not significantly different from those of the registered fungicide Folicur® (tebuconazole) used in these trials. When applied onto wheat cultivars differing in resistance to FHB in field trials in 2009 and 2010, CLO-1 was most effective on the moderately resistant cultivar AC Nass (representing the highest level of resistance commercially available) and least effective on the highly susceptible cultivar AC Foremost. Results of this study suggest that CLO-1 is a promising biocontrol product that may be used in combination with cultivar resistance for managing FHB in wheat.