Abstract
The purpose of this study was to evaluate a protocol for in vitro multiplication of four Musa spp. genotypes. Previous tests indicated the utilization of an ideal benzilaminopurin concentration of 5.0 mg L-1; 5.0 mg L-1; 4.5 mg L-1 e 4.0 mg L-1 in culture medium MS, during the multiplication of Caipira, Thap maeo, PV03-44 and FHIA-01 genotypes, respectively. After that, the multiplication rate, the size of shoots produced per explant, fungal and bacterial contamination rate, oxidation rate, and abnormality occurrence in the multiplication phases were evaluated. The multiplication rate and the size of shoots produced per explant varied among genotypes in the five subcultures studied. The highest contamination rate was caused by bacteria and was more frequent in the in vitro establishment, tending to be reduced with the number of subcultures. The most of the genotypes did not present problems of microorganism contamination, except for Caipira cultivar which presented high bacterial contamination rate during in vitro establishment. This problem is a limitation of the protocol to obtain micropropagated plantlets of this genotype. KEY-WORDS: Musa spp. ; micropropagation; tissue culture; benzilaminopurin.
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