Abstract
To increase the injection volume in capillary zone electrophoresis, a preconcentration step can be carried out. In this way a 50 times higher sample load is easily reached. Two types of such stacking systems are compared by computer simulation and are optimized with respect to pH, effective mobility of the constituents, and lengths of leading and stacking zones. The results of the computer simulation are compared with experiment, and good agreement is found. The application of such an optimized system to the separation of tryptic peptides is demonstrated. High efficiency and good reproducibility are obtained
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