Abstract
ABSTRACT Resonance enhanced coherent anti-Stokes Raman spectrometry (CARS) has been demonstrated as a specific identification system for liquid chromatography. To achieve this work in the areas of CARS. liquid chromatographic preconcentration and separation, and computer control of the liquid chromatograph/ultraviolet-visible/CARS experiments were undertaken. Column selection, flow rate, and solvent composition were monitored and controlled. The ultraviolet-visible spectrometer was used at a fixed wavelength until a species was detected. The flow was stopped, the absorption spectrum was scanned, and flow was resumed. After calculation of the optimum excitation frequency, the Raman spectrum was scanned under control of the computer with on-line signal averaging and data smoothing.
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