Abstract
Two mixtures, one containing model coloured solutes and the other glycosides from the Digitalis species were separated by stepwise gradient thin-layer chromatography, the first in the system silica-toluene + ethyl acetate, the second in the system silica-ethyl acetate + methanol. The optimal gradient programs (i.e., for which maximal spread of spots along the plate is observed) were determined using a computer program reported in an earlier paper. Good separation and satisfactory agreement of predicted and experimental RF values were obtained; the non-aqueous system for the separation of lanatosides permits considerable shortening of elution time owing to the lower viscosity of ethyl acetate-methanol mixtures.
Published Version
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