Abstract
This study focuses on Ultra Violet stress (UVS) gene product which is a UV stress induced protein from cyanobacteria, Synechocystis PCC 6803. Three dimensional structural modeling of target UVS protein was carried out by homology modeling method. 3F2I pdb from Nostoc sp. PCC 7120 was selected as a suitable template protein structure. Ultimately, the detection of active binding regions was carried out for characterization of functional sites in modeled UV-B stress protein. The top five probable ligand binding sites were predicted and the common binding residues between target and template protein was analyzed. It has been validated for the first time that modeled UVS protein structure from Synechocystis PCC 6803 was structurally and functionally similar to well characterized UVS protein of another cyanobacterial species, Nostoc sp PCC 7120 because of having same structural motif and fold with similar protein topology and function. Investigations revealed that UVS protein from Synechocystis sp. might play significant role during ultraviolet resistance. Thus, it could be a potential biological source for remediation for UV induced stress.
Highlights
The continuous depletion of ozone layer results in an increased level of UVB radiation (280–315 nm) that reaches the Earth’s surface and thereby increases the exposure of organisms in surface waters to UVB radiation [1]
Qing-yu Wu [4] had proposed a list of putative UV-B irradiation proteins from cyanobacterium, Synechocystis PCC 6803, identified by MALDI TOF/TOF
Results & Discussion: The target UV-B stress induced protein was obtained from NCBI protein database and the accession was noted as Bioinformation 9(12):639-644 (2013)
Summary
The continuous depletion of ozone layer results in an increased level of UVB radiation (280–315 nm) that reaches the Earth’s surface and thereby increases the exposure of organisms in surface waters to UVB radiation [1]. The response of cyanobacteria to UVB radiation involves a series of damaging effects on DNA, protein and photosynthetic apparatus. It is well known that UVB radiation causes widespread damage in cells and modulates the expression of many genes. Only a few quantitative proteomic studies have explored the impact of damaging solar radiation on microorganisms. A quantitative proteomic approach with 2D gels was used to compare the proteomes of irradiated and unirradiated Deinococcus radiodurans bacteria to identify the mechanisms of their extreme radio-resistance and DNA repair [2, 3] Protein breakdown and recycling, which depend on the levels of proteolytic enzymes, are an essential part of the plant response to environmental stress [4]. In response to environmental abiotic and biotic factors, cellular proteins are normally rebuilt. Degradation of damaged, misfolded and potentially harmful proteins provides free amino acids required for the synthesis of new proteins
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