Computational Modelling of Glucose Uptake in the Enterocyte

David Nickerson,Nima Afshar,Peter J Hunter,Soroush Safaei,Vinod Suresh

doi:10.36903/physiome.13034423.v3

Abstract

We describe an implemented model of glucose absorption in the enterocyte, as previously published by Afshar et al. Afshar et al. (2019), The model used mechanistic descriptions of all the responsible transporters and was built in the CellML framework. It was validated against published experimental data and implemented in a modular structure which allows each individual transporter to be edited independently from the other transport protein models. The composite model was then used to study the role of the sodium-glucose cotransporter (SGLT1) and the glucose transporter type 2 (GLUT2), along with the requirement for the existence of the apical Glut2 transporter, especially in the presence of high luminal glucose loads, in order to enhance the absorption. Here we demonstrate the reproduction of the figures in the original paper by using the associated model. EDITOR'S NOTE (v2): Instructions within the manuscript changed, in order to properly execute the model files. Spelling of author's name corrected in filenames.

Highlights

A validated computational model was proposed for explaining glucose uptake from intestinal lumen into epithelial cell
We present a mathematical model that includes apical glucose transporter type 2 (GLUT2) and is parameterised against published experimental data, and was used to study the contributions of SGLT1 and GLUT2 in published cell culture data on glucose uptake (Zheng et al, 2012)
We constructed a mathematical model of the epithelial cell of a small intestine that incorporates the relevant transport proteins identified in the literature (Barrett and Keely, 2015) and diffusion pathways

Summary

Introduction

A validated computational model was proposed for explaining glucose uptake from intestinal lumen into epithelial cell. The main goal of this paper is to show that the figures in the primary paper can be reproduced by using the correlated model in the PMR. Results from the model were compared with experimental results from Zheng et al (Zheng et al, 2012) which studied the glucose uptake on two different cell lines - Caco and IEC6 - by using varying concentration of glucose (0.5 - 50 mM). We introduce a quick instruction to reproduce each figure in the original paper

Model description

Model results

Discussion