Abstract
Bnm1 is a pollen specific protein from Brassica napus(oilseed rape) and it is specifically expressed in the bi-cellular and tri-cellular stages of pollen development. Since the incidence of pollinosis due to oilseed rape (Brassica napus) is increasing day by day, keeping pace with its high cultivation rate, the search for its allergens is a demand of time to develop effective immune therapy. In the present study, different computational tools were adopted to predict the po tential of Bnm1 as a candidate allergen. Physico chemical properties of Bnm1 showed its molecular weight (~20kD) and theoretical pI (5.27) along with other properties to be fallen between the ranges essential for a protein to be an allergen. Keeping in min d the capability of allergen to induce both humoral and cell mediated immune response, we checked both the potential B cell and T cell epitope candidates of Bnm1 using different immune -informatics tools housed at IEDB analysis resource. For B cell epitopeprediction, potential antigenic sites on the protein surface were predicted by both propensity scale and machine learning method followed by their mapping on Bnm1 3D structure predicted from homology modeling. In case of T cell epitope prediction, interact ion of the core sequence with seven abundant MHC -II alleles (DRB1*0101, DRB1*0301, DRB1*0401, DRB1*0701, DRB1*1101, DRB1*1301 and DRB1*1501) within an IC50 range (IC50<25) was the basis. We observed multiple epitope interactions with DRB1*0101 allele, impl ying that this interactions might evoke a strong TH2 response ensuring an increased production of IgE response. Based on our prediction we hereby claim that the Bnm1 is a potential allergen having capability to induce both humoral and cell mediated allergi c responses. However, in vitro analysis was out of our scope and therefore it should have to be performed to validate the potency of Bnm1 as an allergen.
Highlights
Allergens are mostly small proteins or protein bound small substances having a molecular weight ranging from 15 to 40 kD [1]
2.4 Potential B cell epitope prediction: All the regions exposed to outer surface are not potent site to react with B cell, that’s why B cell epitopes are needed to be predicted
Our result suggests that Bnm1 protein may induce production of IgE response which is largely associated with HLADRB1*01:01 allele
Summary
Allergens are mostly small proteins or protein bound small substances having a molecular weight ranging from 15 to 40 kD [1]. Brassica napus (oilseed rape), belonging to cruciferae family, is one of the most cultivated crop plants around the world. Pollens of Brassica napus guided by the wind are spreaded everywhere around the cultivated land and people mostly farmers having exposed to it suffer from hay-fever, allergic rhinitis like seasonal diseases [15]. In silico approaches have made it possible to give a straightforward time and money saving way to find out the solution of many biological problems like vaccine design [21], prediction of deleterious effects of mutations [22] and many more It can predict potential allergens from a given whole proteome. This is the first immune-informatics approach to examine the potency of Bnm from oilseed rape to be an allergen
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: International Journal on Bioinformatics & Biosciences
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
