Computational and biological efficacy of stigmasterol against HeLa cells and Vero cells- first time isolated from the ethanolic extract of Annonamuricata Linn leaves

Abstract

The present study was aimed to analyze the in vitro antioxidant, anti-inflammatory and cytotoxicity activities of isolated bioactive compound stigmasterol (abbreviated as STML) from the leaves of Annonamuricata. Extraction was done using Soxhlet apparatus, TLC, column chromatography and STML in the extracts were identified by Gas-Chromatography and Mass-Spectroscopy (GC–MS) analyses. Characterizations of isolated bioactive compound stigmasterol for purity verified were performed using High-performance liquid chromatography (HPLC) technique. Stigmasterol compound was estimated by DPPH scavenging and ABTS assay, it showed significant values. In vitro cytotoxicity activity of stigmasterol was also tested HeLa cells and Vero cells and IC50 values found that 11.58 µg/ml and 173.8 µg/ml, respectively. The different spectral analysis were also characterized by experimental and well deal with the theoretical ab initio Density functional theory (DFT) method at B3LYP level with 6–311++G(d,p) basis set of provides for the different spectral studies, respectively by using UV-Visible,1Hand 13C nuclear magnetic resonance (NMR) spectroscopy studies. Fourier-transform infrared (FTIR) spectral analysis was carried out. Density Function Theory (DFT) computations enabled us to get different reactive properties of STML. The Rf of STML isolated from the ethanolic extracts of Annonamuricata leaves is 3.707. In conclusion, the results suggest that the investigated compounds are potential drug leads to target HeLa cells and Vero cells.