Abstract

Hyaluronidases are a group of glycosidases enzymes, which are widely produced by different organisms. To find a bacterial source of hyaluronidases with promising industrial characteristics, different species of Bacillus were analyzed according to their various physiochemical properties, multiple sequence alignment, phylogenetic tree construction, homology search for motifs, and structural and functional properties via various computational approaches. The evaluation showed that the hyaluronidase of B. selenitireducens with the highest molecular mass, a high level of thermostability and solubility, and the phylogenetic relationship to hyaluronidas derivative from S. aureus can be considered as a potential candidate for biotechnological applications. Generally, the present study provides a detailed overview of functional, and structural characteristics as well as phylogenetic relation of Bacillus hyaluronidases, which could be helpful for future investigations in order to find the best bacterial hyaluronidase.

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