Computational Analysis between two different hemoglobins from Caspian Sea sturgeons (Acipenser persicus and Acipenser stellatus) induced by n‐dodecyl trimethylammonium bromide

Abstract

The multiplicity of the Hemoglobins (Hbs) of the and the A. stellatus, two species from the Caspian Sea, A.persicus was confirmed with the polyacrylamide gel electrophoresis (SDS‐PAGE), cellulose acetate electrophoresis, and isoelectric focusing (IEF) experiments. In the current study, the interaction of n‐dodecyl trimethylammonium bromide (DTAB) as a cationic surfactant under variable concentrations, with the purified dominant Hbs was investigated using UV‐visible absorption spectra and circular dichroism (CD). The chemometric resolution techniques were used to determining the number of the components and mole fraction of the oxidized Hbs. The analysis of the obtained spectral data using singular value decomposition (SVD), evolving factor analysis (EFA) and multivariate curve resolution‐alternative least square (MCR‐ALS) as well‐known chemometrical techniques, gave the evidence for the existence of four different molecular components including native (N), intermediate (I), intermediate (II) and denatured (D) in sturgeon Hbs. On the basis of the interpretation of chemometrical results, the formation of intermediate states of fish Hbs, plays an important role for regulation of the respiratory system of the fishes proportional to their habitat depths and environmental partial pressure of oxygen.