Abstract
We explain how volumetric light-field excitation can be converted to a process that entirely avoids 3D reconstruction, deconvolution, and calibration of optical elements while taking scattering in the probe better into account. For spatially static probes, this is achieved by an efficient (one-time) light-transport sampling and light-field factorization. Individual probe particles (and arbitrary combinations thereof) can subsequently be excited in a dynamically controlled way while still supporting volumetric reconstruction of the entire probe in real-time based on a single light-field recording.
Highlights
Volumetric recording of fluorescent activity is an essential tool in modern microscopy
Light-Field Microscopy (LFM)[7,11,12], in contrast, captures the 4D incident light (e.g. by means of a microlens array (MLA) placed at the intermediate image plane of the imaging path), and supports the computation of a focal stack based on a single sensor recording
We explain how volumetric light-field excitation (VLE) can be converted to a process that entirely avoids 3D reconstruction, deconvolution, and calibration of optical elements while taking scattering in the probe better into account
Summary
Equation 1 outlines the principle of compressive volumetric light-field excitation (CVLE):. To achieve an additional speed-up, we consider the isotropic emission of the probe and avoid subdivisions down to the last (single-ray) level: We hypothesize that isotropically emitting particles cause the same imaging light-field signature (pattern) when hit by different illumination rays. In this case, 179 frames must be scanned for 8085 illumination rays to estimate a light-transport matrix that is close to the ground truth (i.e., the result of brute-force sampling). In our implementation we apply an active-set approach to alternating non-negative least squares[32]
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