Abstract
We have developed compressed sensing single pixel spatial frequency domain imaging (cs-SFDI) to characterize tissue optical properties over a wide field of view ( 35 ?? mm × 35 ?? mm ) using multiple near-infrared (NIR) wavelengths simultaneously. Our approach takes advantage of the relatively sparse spatial content required for mapping tissue optical properties at length scales comparable to the transport scattering length in tissue ( l tr ? 1 ?? mm ) and the high bandwidth available for spectral encoding using a single-element detector. cs-SFDI recovered absorption ( ? a ) and reduced scattering ( ? s ? ) coefficients of a tissue phantom at three NIR wavelengths (660, 850, and 940 nm) within 7.6% and 4.3% of absolute values determined using camera-based SFDI, respectively. These results suggest that cs-SFDI can be developed as a multi- and hyperspectral imaging modality for quantitative, dynamic imaging of tissue optical and physiological properties.
Highlights
Spatial frequency domain imaging (SFDI) is a noncontact wide-field imaging technique that uses sinusoidal patterns of intensity-modulated light to characterize multiply scattering media, such as biological tissue
We constructed tissue phantoms to investigate the ability of the cs-SFDI instrument to estimate bulk optical properties.[19]
The “sample” phantom consists of two regions: a central lesion, which is made of Naphthol Green B dye as the main absorber, and TiO2 as the main scatterer both dispersed in a cured polydimethylsiloxane (PDMS) base
Summary
Spatial frequency domain imaging (SFDI) is a noncontact wide-field imaging technique that uses sinusoidal patterns of intensity-modulated light to characterize multiply scattering media, such as biological tissue. SFDI separates the contributions of light scattering from absorption by measuring the frequency-dependent modulation transfer function of diffusively reflected structured light. This information is used to map and form images of tissue optical parameters μs[0] and μa, respectively.[1,2] Optical properties at multiple wavelengths are used to derive quantitative images of biochemical composition,
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