Abstract
Members of the subtribe Lychnophorinae occur mostly within the Cerrado domain of the Brazilian Central Plateau. The relationships between its 11 genera, as well as between Lychnophorinae and other subtribes belonging to the tribe Vernonieae, have recently been investigated upon a phylogeny based on molecular and morphological data. We report the use of a comprehensive untargeted metabolomics approach, combining HPLC-MS and GC-MS data, followed by multivariate analyses aiming to assess the congruence between metabolomics data and the phylogenetic hypothesis, as well as its potential as a chemotaxonomic tool. We analyzed 78 species by UHPLC-MS and GC-MS in both positive and negative ionization modes. The metabolic profiles obtained for these species were treated in MetAlign and in MSClust and the matrices generated were used in SIMCA for hierarchical cluster analyses, principal component analyses and orthogonal partial least square discriminant analysis. The results showed that metabolomic analyses are mostly congruent with the phylogenetic hypothesis especially at lower taxonomic levels (Lychnophora or Eremanthus). Our results confirm that data generated using metabolomics provide evidence for chemotaxonomical studies, especially for phylogenetic inference of the Lychnophorinae subtribe and insight into the evolution of the secondary metabolites of this group.
Highlights
Vernonieae contains 21 currently recognized subtribes [1,2,3,4]
Aqueous-methanol extracts were prepared from dried leaves and UHPLC-MS based metabolic fingerprinting was performed for all species, in both positive and negative electrospray ionization (ESI) modes
Dichloromethane extracts were prepared from dried leaves and GC-MS-based metabolic fingerprinting was performed for all species, in an electron ionization (EI) mode
Summary
Vernonieae contains 21 currently recognized subtribes [1,2,3,4]. The subtribe Lychnophorinae is nearly endemic to Brazil [1,2,5,6]. 120 species [1,2,5,6]. Most species are restricted to campo rupestre (literally rocky fields) in the highlands of southeastern and northeastern Brazil and to the Cerrado domain (Brazilian savanna). From a phytochemical point of view, these species exhibit high diversity of compounds.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
