Comprehensive study of sporicidal and sporstatic effect of CuO and AgO metal nanoparticles upon spore of Clostridium botulinum type E

Abstract

Nanoparticles due to high volume levels have high sporicidal and bacterial properties. In this study, we examined the sporicidal effect of metal oxide nanoparticles of silver and copper on the bacterium Clostridium botulinum type E. In this study, the bacterium, C. botulinum type E, and silver and copper oxide nanoparticles with average diameter of 20 nm were employed. The bacterium was identified and confirmed by PCR method. All colors and media culture used were of Merck Company. The bacterium was determined and confirmed by PCR and antibody was raised against C. botulinum type E. Peterson method was used for isolation and purification of spores. Ratios of MIC/MBC and phenol coefficient were calculated by microdilution method; and Syndics’ sporicidal obtained amount of D value was determined by pure plate method. Strains of bacteria were confirmed by catalytic domain of C. botulinum type E. SEM and TEM images confirm nanoparticles size was about 20 nm. Powers of sporicidal effect of the nanoparticles were compared with other chemical sporicidals, like Glutaraldehyde. Phenol coefficient obtained was about 50 and the ratio of MIC/MBC good was about 1/2, respectively. D value for the critical concentration of silver nanoparticles was about 7 min. Results of this study showed that nanoparticles studied for its high ratio of surface to volume properties had high sporicidal effect and it is predicted that this nanoparticle can be used for environmental sterilization.